Media Preparation
- Preparing Media (general guide, malt extract agar)
- V8 Agar
- MSR media (Cranenbrouck et al., in Declerck et al. 2005, In Vitro Culture of Mycorrhizas)
- Parafilming plates (When parafilming goes wrong: how to salvage a Situation)
Molecular Techniques/Resources
- Qubit fluorometric quantitation (Fisher user assets)
- Basic PCR protocol (New England Biolabs)
- Calculator for Resuspending Oligos/Custom Primers (Thermo Fisher)
- How to resuspend and dilute primers (Thermo Fisher protocols; primers should be resuspended at 100x in molecular grade H20 for storage at -20, in aliquots so as to minimize freeze-thaw cycles. Working dilutions are made in molecular grade water 10:90)
- Troubleshooting gel electrophoreisis (BentoBio Knowledge Hub guide)
- PCR Workbook
- Using a Micropipette
Culture Techniques
- Surface Sterilization (for endophyte culturing)
- Clearing and Staining Roots
- Establishing Monotypic AMF cultures
- AMF isolation: ultrasound centrifugation technique (protocol development by Shana Mann et Klymiuk, after Boyno et al. 2023)
- Seed sterilization for monoxenic culture (for hosting AMF)
Field Work and Outdoor Safety
Klymiuk Laboratory for Plant Mycobiomes 